![]() Staining allows different structural components of the cells to be visualized including the cytoplasm, cell wall, and membranes. When looking at bacteria under the microscope much of the bacteria can appear transparent without staining. Mix the bacteria on the loop with the water you placed on the glass slide earlier and then flame your loop once again.Remember to maintain good aseptic technique to prevent contamination of the sample. Next, immediately remove a bacteria colony from the agar plate or broth using the loop.Allow the loop to glow red and then cool. Firstly, place the inoculation loop into the blue portion of the flame of the bunsen burner. The smear is now ready to be prepared.Using an inoculating loop, place a small drop of water whee you are going to place the bacteria and perform the smear.Using your felt marker, place a small mark where you will be placing the bacteria and making the smear.This involves placing a thin layer of bacteria onto a glass slide, typically before staining it. In order to do this, a bacterial smear must be performed. Once the bacteria has been left to incubate and grow on the agar plate, it then is viewed under the microscope. For example, blood agar is commonly used to identify bacteria that are responsible for hemolysis of the blood e.g. Differential Culture Media – This is a kind of media that is used to differentiate between bacteria.Selective Agar -This a type of agar that inhibits the growth of certain bacteria while allowing the growth of another e.g. Mannitol salt agar is selective for Gram-positive bacteria. ![]() This includes aerobic, anaerobic and microaerophilic microorganisms. Nutrient Agar media – This is the most common type of nutrient media used and is a non-selective media that allows the growth of a wide variety of different species.These are some common types of culture media used in modern microbiology laboratories: Since bacteria have different nutrient requirements, the exact type of media used depends on the bacteria that is being selected for. Microscope Bacteria Preparation Culturing Bacteria Speciesīefore certain bacteria can be seen under a light microscope, they first must be grown in a nutrient-rich culture media. What Type of Microscope is Best for Bacteria Observation?.Examples of Bacteria Under the Microscope. ![]()
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